Coding
ADC_Y21W,

Part:BBa_K590041

Designed by: Juhye An, Benjamin Mo   Group: iGEM11_Washington   (2011-09-15)

Aldehyde Decarbonylase_Y21W, C70I

[http://2011.igem.org/Team:Washington University of Washington 2011 iGEM Team] has designed this mutant Aldehyde Decarbonylase (ADC) to modify the specificity of the wild type ADC so that when it works together with Acyl-ACP Reductase (AAR), it will produce more C13 and C15 alkanes. For more information, refer to the fully assembled brick, BBa_K590058.

Usage and Biology

This mutant Aldehyde Decarbonylase has amino acid changes from tyrosine, Y, to tryptophan, W, at residue 21 and from cysteine, C, to isoleucine, I, at residue 70.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 730
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None